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MICROBIOME!

Microbiome sequencing is transforming how we understand the invisible ecosystems that impact health, environment, and industry. At MR DNA (www.mrdnalab.com), we harness the latest sequencing technologies to provide comprehensive insights into microbial communities across diverse environments. From human gut microbiomes to soil and aquatic ecosystems, our advanced platforms uncover the intricate balance of bacteria, archaea, fungi, and other microorganisms that drive biological processes.

Our microbiome sequencing services include 16S rRNA, ITS, and metagenomic approaches, offering unparalleled resolution and versatility. By targeting key genetic markers, we can identify microbial diversity and functions with precision, while metagenomics allows us to explore functional genes and metabolic pathways in detail. Whether you need to monitor health-related microbial shifts, analyze environmental biodiversity, or optimize industrial microbiology processes, MR DNA delivers results you can trust.

What sets MR DNA apart is our dedication to customization and quality. With flexible primer options and expert bioinformatics support, we tailor every project to your specific research needs. Our streamlined workflows and in-depth reports provide actionable insights, empowering you to make data-driven decisions. Partner with us to unlock the potential of microbiome science—visit www.mrdnalab.com today to learn how our sequencing services can transform your research and innovation.

Here is a detailed list of microbiome research primers, including sequences where available, grouped by their target regions.

16S rRNA Primers (Bacteria & Archaea)

  1. 27F: AGAGTTTGATCMTGGCTCAG / 1492R: GGTTACCTTGTTACGACTT
  2. 8F: AGAGTTTGATCCTGGCTCAG / 534R: ATTACCGCGGCTGCTGG
  3. 515F: GTGCCAGCMGCCGCGGTAA / 806R: GGACTACHVGGGTWTCTAAT
  4. 341F: CCTACGGGNGGCWGCAG / 805R: GACTACHVGGGTATCTAATCC
  5. 338F: ACTCCTACGGGAGGCAGCAG / 907R: CCGTCAATTCMTTTRAGTTT
  6. 27F: AGAGTTTGATCMTGGCTCAG / 534R: ATTACCGCGGCTGCTGG
  7. 515F: GTGCCAGCMGCCGCGGTAA / 926R: CCGTCAATTCMTTTRAGTTT
  8. 63F: CAGGCCTAACACATGCAAGTC / 1387R: GGGCGGWGTGTACAAGGC
  9. 519F: CAGCMGCCGCGGTAA / 785R: TTACTCCTGGGCTTGCT
  10. 799F: AACMGGATTAGATACCCKG / 1193R: ACGTCATCCCCACCTTCC

ITS Primers (Fungi)

  1. ITS1: TCCGTAGGTGAACCTGCGG / ITS2: GCTGCGTTCTTCATCGATGC
  2. ITS1F: CTTGGTCATTTAGAGGAAGTAA / ITS2: GCTGCGTTCTTCATCGATGC
  3. ITS1F: CTTGGTCATTTAGAGGAAGTAA / ITS4: TCCTCCGCTTATTGATATGC
  4. ITS3: GCATCGATGAAGAACGCAGC / ITS4: TCCTCCGCTTATTGATATGC
  5. ITS1F: CTTGGTCATTTAGAGGAAGTAA / ITS4B: CAGGAGACTTGTACACGGTCCAG
  6. ITS2: GCTGCGTTCTTCATCGATGC / ITS3: GCATCGATGAAGAACGCAGC
  7. ITS5: GGAAGTAAAAGTCGTAACAAGG / ITS4: TCCTCCGCTTATTGATATGC
  8. ITS1: TCCGTAGGTGAACCTGCGG / ITS4: TCCTCCGCTTATTGATATGC
  9. ITS1F: CTTGGTCATTTAGAGGAAGTAA / ITS5: GGAAGTAAAAGTCGTAACAAGG
  10. ITS1F: CTTGGTCATTTAGAGGAAGTAA / ITS2-KYO1: TAGAGGAAGTAAAAGTCGTAA

Archaea-Specific Primers

  1. 21F: TTCCGGTTGATCCYGCCGGA / 958R: YCCGGCGTTGAMTCCAATT
  2. 344F: ACGGGGYGCAGCAGGCGCGA / 915R: GTGCTCCCCCGCCAATTCCT
  3. 524F-Arch: TGYCAGCCGCCGCGGTAAHACCVGC / 958R-Arch: YCCGGCGTTGAMTCCAATT
  4. 571F: GCCAGCAGCCGCGGTAA / 915R: GTGCTCCCCCGCCAATTCCT
  5. 349F: GYGCASCAGKCGMGAAW / 806R: GGACTACNSGGGTMTCTAAT
  6. 517F: GCYTAAAGSRNCCGTAGC / 958R: YCCGGCGTTGAMTCCAATT
  7. 519F: CAGCMGCCGCGGTAA / 806R: GGACTACNSGGGTMTCTAAT
  8. 340F: CCTACGGGGGCGCAG / 1000R: GGGCATCGTTTACGGG
  9. 524F: TGYCAGCCGCCGCGGTAAHACCVGC / 958R: YCCGGCGTTGAMTCCAATT
  10. 344F: ACGGGGYGCAGCAGGCGCGA / 915R: GTGCTCCCCCGCCAATTCCT

Functional Genes

  1. nirS: CCTAYTGGCCGCCRCART / nirK: GCCTCGATCAGRTTRTGGTT
  2. amoA: GGGGTTTCTACTGGTGGT / amoA-R: CCCCTCKGSAAAGCCTTCTTC
  3. dsrA: ACSCACTGGAAGCACG / dsrB: GTGGMRCCGTGCAKRTTGG
  4. mcrA: GGTGGTGTMGGATTCACACARTAYGCWACAGC / mcrA-R: TCCAGGAACTACGAGTTTTWTCWTASWAACCA
  5. pmoA: AAYTGGGGCATCCTTCC / pmoA-R: GAACGTCCTTACCGT
  6. nifH: TGYGAYCCNAARGCNGA / nifH-R: ADSAWYCCAACATYTTCCAC
  7. hzo: GGNTGYTAYACNGCNGGNTAYCC / hzo-R: TAYTTNGCNGGYTGYTGRTGNACCCA
  8. soxB: TAAYTGGCAGGGATGCTT / soxB-R: TGNCCYTCRTGRCARTGGCAT

This list incorporates a variety of primer sets, suitable for bacterial, archaeal, fungal, and functional gene studies. Let me know if you need additional details or further expansion!